Coding

Part:BBa_K2287027:Design

Designed by: Jiang Wu   Group: iGEM17_UCAS   (2017-10-20)


purF K326Q


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 876
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 94
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The mutagenesis from AAA to CAG (979-981) was made based on E.coli codon bias.


Source

This original sequence can directly obtained by PCR from genomic DNA of E.coli strain MG1655. Specialized primers were designed to do site-directed mutagenesis by PCR.

References